- Cast: Laurence R. Harvey, Ashlynn Yennie, Dominic Borrelli, Georgia Goodrick, Lucas Hansen, Emma Lock, Dan Burman, Bill Hutchens, Maddi Black, Katherine Templar, Lee Nicholas Harris, Peter Blankenstein, Kandace Caine, Daniel Jude Gennis, Vivien Bridson, Peter Charlton, Daniel De'sioye
- Director: Tom Six
- Writer: Tom Six
- Producers: Tom Six, Ilona Six
- D.O.P.: David Meadows
- Makeup: Harriet Thompson
- Music: James Edward Barker
The story of a man who becomes sexually obsessed with a DVD recording of the first film in the series, The Human Centipede, and uses sandpaper to pleasure himself whilst watching the film. He decides to create a "human centipede" of his own, this time comprised of twelve victims as opposed to the first film's three.
He uses his human centipede to his own sexual delight, taking sexual pleasure in watching the victims of the centipede defecate in each other's mouths, and using barbed wire wrapped around around his penis to rape the woman at the end of the centipede.
- Aspect Ratio: 1.85:1
- Filming Locations: London, UK
- Production Companies: Six Entertainment
- Production Schedule: June 2010